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Can I read Functional alterations of G-proteins in diabetic rat retina: a possible explanation for the early visual abnormalities in diabetes mellitus on EtoBox?
Functional alterations of G-proteins in diabetic rat retina: a possible explanation for the early visual abnormalities in diabetes mellitus by A. Kowluru; R. A. Kowluru; A. Yamazaki is a Medicine article available to read on EtoBox.
What is Functional alterations of G-proteins in diabetic rat retina: a possible explanation for the early visual abnormalities in diabetes mellitus about?
We examined changes in guanosine triphosphatedependent signal transduction mechanisms in the retina from the early stages of the streptozotocin-diabetic rat, a model for Type I (insulin-dependent) diabetes mellitus. Guanosine triphosphate binding, guanosine triphosphatase activity, and binding of (azido) guanosine triphosphate decreased significantly in the retina as early as 2 weeks after the induction of diabetes. The ability of guanosine triphosphate to inhibit forskolin-stimulatable adenyl cyclase was also abolished. These data suggest functional deterioration of G-proteins, especially Gi, in diabetic retina. Further studies using retinal rod outer segments revealed deterioration in light-sensitive, guanosine triphosphate-dependent functions of transducin in diabetic rats. Pertussis toxin-catalysed ADP ribosylation of the ~z subunit of transducin, a he-terotrimeric G-protein of rod outer segments, was also reduced in diabetes. No functional effects were seen in purified snbunits of transducin subjected to non-enzymatic glycation in vitro. On the other hand, incubation of non-diabetic rod outer segments with (12-0-tetradeconyl) phorbol-13-acetate, a protein kinase C agonist, in
Who reads Functional alterations of G-proteins in diabetic rat retina: a possible explanation for the early visual abnormalities in diabetes mellitus?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- A. Kowluru; R. A. Kowluru; A. Yamazaki
- Publisher
- Springer; Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC (ISSN 0012-186X)
- Published
- 1992
- Language
- EN
- Field
- Medicine (Health Sciences)