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Structural analysis of the heptose/hexose region of the lipopolysaccharide from escherichia coli K-12 strain W3100 by Otto Holst; Ulrich Zähringer; Helmut Brade; Aleksander Zamojski is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Structural analysis of the heptose/hexose region of the lipopolysaccharide from escherichia coli K-12 strain W3100 about?

The disaccharide L-glycero-D-manno-heptosyl-D-glucose was isolated from the lipopolysaccharide (LPS) of Escherichia coli K-12 strain W3100 after partial hydrolysis with acid, and the structure was determined by methylation analysis, n.m.r. spectroscopy, and comparison with a synthetic standard. In addition, the oligosaccharides L,D-Hep-D-Glc-D-Glc and L,D-Hep-D-Glc-D-Glc-D-Glc were isolated, and their structures were established by g.l.c.-m.s. and methylation analysis. The results indicated that L-glycero-D-manno-heptose, a characteristic constituent of the inner core region, may also occur in the outer core region which, in E. coli, is generally composed of hexoses. A revised structure of the carbohydrate backbone of the hexose/heptose region of the LPS is given.

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Author

Otto Holst; Ulrich Zähringer; Helmut Brade; Aleksander Zamojski

Publisher

Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0008-6215)

Published

1991

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)