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Effects of tranexamic acid on the activity of glutamate transporter EAAT3 by Hyun-Jung Shin; Soo-Young Lee; Hyo-Seok Na; Bon-Wook Koo; Jung-Hee Ryu; Sang-Hwan Do is a Medicine article available to read on EtoBox.
What is Effects of tranexamic acid on the activity of glutamate transporter EAAT3 about?
## Background Tranexamic acid (TXA) is the most widely used hemostatic agent in surgical patients. However, when used in a high dose, it could cause a seizure in the postoperative period. The exact effector mechanism behind the seizure triggering remains unknown. Therefore, the authors investigated the effects of TXA on the activity of glutamate transporter type 3 (excitatory amino acid transporter 3; EAAT3), which is the main neuronal glutamate transporter type. ## Methods EAAT3 was expressed in __Xenopus laevis__ oocytes through mRNA injection. Oocytes were incubated with diluted tranexamic acid for 72 h. Two-electrode voltage clamping was used to measure membrane currents before, during, and after applying 30 μM L-glutamate. Responses were quantified by integrating the current traces and reported in microcoulombs (μC). Results were presented as mean ± SEM. ## Results TXA (30 to 1,000 μM) significantly decreased EAAT3 activity. Our kinetic study showed that V~max~ was significantly decreased in the TXA group compared with the control group (1.1 ± 0.1 vs. 1.4 ± 0.1 μC, n = 18–23, P = 0.043), but the K~m~ did not significantly change (12.7 ± 3.9 μM for TXA vs. 12.8 ± 3.8 for contro
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- Author
- Hyun-Jung Shin; Soo-Young Lee; Hyo-Seok Na; Bon-Wook Koo; Jung-Hee Ryu; Sang-Hwan Do
- Publisher
- The Editorial Office of Anesthesia and Pain Medicine
- Published
- 2020
- Language
- EN
- Field
- Medicine (Health Sciences)