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Enrichment of N-3 Polyunsaturated Fatty Acids Into Acylglycerols of Borage Oil via Lipase-catalyzed Reactions Under Supercritical Conditions by Tsao-Jen Lin; Shin-Wan Chen is a Engineering article available to read on EtoBox.
What is Enrichment of N-3 Polyunsaturated Fatty Acids Into Acylglycerols of Borage Oil via Lipase-catalyzed Reactions Under Supercritical Conditions about?
Esterification of free n-3 polyunsaturated fatty acid (PUFA) with enriched n-6 triglyceride (TG) was catalyzed to modify the desired structured lipid with n-3/n-6 = 4 by using lipase under supercritical carbon dioxide (SCCO 2 ). Comparing four different types of lipases, 1,3-specific lipase from Mucor miehei has the highest degree of incorporation under 10 wt.% loading amount of total substrates. The optimal operating parameters under 10.2 MPa and 323.15 K SCCO 2 can attain the desired n-3/n-6 ratio in 6 h. Because of the negative effect on the enzyme activity by the enriched n-6 TG, the optimal substrate ratio of the enriched n-6 TG and the n-3 PUFA was chosen as 1/4. To enhance the solubility of n-3 PUFA in SCCO 2 , ethanol was applied as a co-solvent and reached an optimal input at 10 wt.% of total substrates. The activity of the enzyme still maintained 81% of initial activity because of de-moisture from the surface enzyme after seven cyclic pressurization/depressurizations. A model of the ping-pong mechanism and the substrate inhibition was proposed to express the catalytic action of lipase. With the aid of the Michaelis-Menten equation and Lineweaver-Burk plot, the estimated r
Who reads Enrichment of N-3 Polyunsaturated Fatty Acids Into Acylglycerols of Borage Oil via Lipase-catalyzed Reactions Under Supercritical Conditions?
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Tsao-Jen Lin; Shin-Wan Chen
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1385-8947)
- Published
- 2008
- Language
- EN
- Field
- Engineering (Physical Sciences)