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Automated Immunoassay Methods for Ferritin: Recovery Studies to Assess Traceability to an International Standard by Sheena Blackmore; Malcolm Hamilton; Anne Lee; Mark Worwood; Matthew Brierley; Alan Heath; Susan J. Thorpe is a Medicine article available to read on EtoBox.
What is Automated Immunoassay Methods for Ferritin: Recovery Studies to Assess Traceability to an International Standard about?
Background: Ferritin standardisation is problematical due to the heterogeneity of ferritin isoforms and the antibodies used in its immunoassay, and the lack of a reference measurement procedure. We investigated the performance of the 1st (liver), 2nd (spleen) and 3rd (recombinant) International Standards (ISs) for ferritin in major assays. Methods: The ferritin in a serum pool 'spiked' with either the 2nd or 3rd IS for ferritin was measured by 52 laboratories using five automated methods and the recovery of the target values calculated. A smaller serum pool was 'spiked' with the 1st IS for a limited recovery exercise. The ferritin values of five serum samples were also measured and recalculated relative to the ISs. Results: Recoveries of each of the 2nd and 3rd ISs were 90%-110% for four of five methods; recoveries of the 1st IS were 104% and 111% for two of three methods claiming traceability to this IS. One method significantly over-recovered each of the IS (124%-155%). Recalculating the ferritin values of the serum samples relative to the IS reduced the overall inter-method agreement, largely because of the anomalous over-recovery of the IS by one method. Conclusions: The use of
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- Author
- Sheena Blackmore; Malcolm Hamilton; Anne Lee; Mark Worwood; Matthew Brierley; Alan Heath; Susan J. Thorpe
- Publisher
- Walter de Gruyter GmbH
- Published
- 2008
- Language
- EN
- Field
- Medicine (Health Sciences)