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Lower-dimensional projections of cellular expression improves cell type classification from single-cell RNA sequencing by Umar, Muhammad; Asif, Muhammad; Mahmood, Arif is a scholarly article available to read on EtoBox.

What is Lower-dimensional projections of cellular expression improves cell type classification from single-cell RNA sequencing about?

Single-cell RNA sequencing (scRNA-seq) enables the study of cellular diversity at single cell level. It provides a global view of cell-type specification during the onset of biological mechanisms such as developmental processes and human organogenesis. Various statistical, machine and deep learning-based methods have been proposed for cell-type classification. Most of the methods utilizes unsupervised lower dimensional projections obtained from for a large reference data. In this work, we proposed a reference-based method for cell type classification, called EnProCell. The EnProCell, first, computes lower dimensional projections that capture both the high variance and class separability through an ensemble of principle component analysis and multiple discriminant analysis. In the second phase, EnProCell trains a deep neural network on the lower dimensional representation of data to classify cell types. The proposed method outperformed the existing state-of-the-art methods when tested on four different data sets produced from different single-cell sequencing technologies. The EnProCell showed higher accuracy (98.91) and F1 score (98.64) than other methods for predicting reference fr

Author

Umar, Muhammad; Asif, Muhammad; Mahmood, Arif

Published

2024

Language

EN