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Improved high-performance liquid chromatographic analysis of methylated amino acids and related compounds and its application to the analysis of samples of polluted healthy and damaged Picea needles without extract purification by V.R. Villanueva; M. Mardon; M.Th. Le Goff; F. Moncelon is a Chemistry article available to read on EtoBox.
What is Improved high-performance liquid chromatographic analysis of methylated amino acids and related compounds and its application to the analysis of samples of polluted healthy and damaged Picea needles without extract purification about?
A fully automated, rapid and sensitive method for the separation of 24 interrelated amino compounds is presented. It uses a high-performance laboratory-made analyser, ion-exchange chromatography and fluorimetric with o-phthalaldehyde detection. All methylated known natural derivatives of histidine, lysine and arginine are well separated. The method uses three buffers and a single column temperature. An analysis requires 118 min and the method is sensitive to picomole levels of the compounds concerned. Ion-exchange chromatography and the composition of the buffers allow the application of the method to samples from different biological sources without previous purification, thus saving time and avoiding losses of amino compounds, particularly those present in trace amounts. It has been used for the analysis of crude polluted Picea needle samples.
Who reads Improved high-performance liquid chromatographic analysis of methylated amino acids and related compounds and its application to the analysis of samples of polluted healthy and damaged Picea needles without extract purification?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- V.R. Villanueva; M. Mardon; M.Th. Le Goff; F. Moncelon
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)
- Published
- 1987
- Language
- EN
- Field
- Chemistry (Physical Sciences)
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