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Identification of Cat Sequences Required for Intron‐dependent Gene Expression in Maize Cells by Nikki Rethmeier; Evelien Kramer; Marc Va; N Montagu; Marc Cornelissen is a Agricultural and Biological Sciences article available to read on EtoBox.

What is Identification of Cat Sequences Required for Intron‐dependent Gene Expression in Maize Cells about?

## Summary Transgene expression in maize cells changed from intron‐independent to intron‐dependent by an exact exchange of the __bar__ coding region for that of __cat__. By deletion mapping an approximately 100 nucleotide sequence element at the 5′ end of the __cat__ coding region was identified that, when inserted at the translation start site of the __bar__ gene, impaired expression. Successive inclusion of the __salT__ intron in the 5′ untranslated region (UTR) restored expression near to wild‐type __bar__ expression levels. A chimeric __gfp__ gene, but not __npt__II gene, behaved similarly. These observations are in agreement with the view that intron‐mediated enhancement of transgene expression does not enhance, but rather restores expression of an impaired gene.

Who reads Identification of Cat Sequences Required for Intron‐dependent Gene Expression in Maize Cells?

Author

Nikki Rethmeier; Evelien Kramer; Marc Va; N Montagu; Marc Cornelissen

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0960-7412)

Published

1998

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)