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Role of the nucleoid-associated proteins governing the interaction between host chromosome and carbazole degradative plasmid pCAR1 by Chiho Suzuki; Choong-Soo Yun; Tohru Terada; Kazuya Watanabe; Hisakazu Yamane; Hideaki Nojiri is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Alkane 1-monooxygenase (alkB); n-Alkanes; Rhodococcus; Beta-galactosidase Rhodococcus sp. BCP1 grows on gaseous (C 2 -C 4 ), liquid (C 5 -C 16 ) and solid (C 17 -C 28 ) alkanes and it can also co-metabolize low-chlorinated solvents under aerobic conditions. A chromosomal region, including the alkB gene cluster, was cloned from Rhodococcus sp. BCP1 genome. The alkB gene cluster is homologue to the gene cluster family encoding an integral-membrane non-heme di-iron monooxygenase (alkB), two rubredoxins and rubredoxin reductase, acting as electron-transfer proteins. Retro-transcriptase-PCR analysis revealed that alkB gene cluster is harboured on a polycistronic operon whereas the transcription start of alkB was determined by primer extension procedure. In order to clarify the range of the substrates inducing the alkane hydroxylase expression, cells of Rhodococcus sp. BCP1 were transformed with a promoter probe vector containing the lacZ gene downstream of the alkB promoter. Resting cells of Rh. sp. BCP1 showed high level of ␤-galactosidase activity induced by the growth on a range of n-alkanes comprised between hexane (C 6 ) and eicosane (C 20 ). By contrast, butane (C 4 ) and pentane

Who reads Role of the nucleoid-associated proteins governing the interaction between host chromosome and carbazole degradative plasmid pCAR1?

Author

Chiho Suzuki; Choong-Soo Yun; Tohru Terada; Kazuya Watanabe; Hisakazu Yamane; Hideaki Nojiri

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0168-1656)

Published

2010

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)