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Can I read Human immunodeficiency virus serotyping on dried serum spots as a screening tool for the surveillance of the AIDS epidemic on EtoBox?
Human immunodeficiency virus serotyping on dried serum spots as a screening tool for the surveillance of the AIDS epidemic by Francis Barin; Jean-Christophe Plantier; Denys Brand; Sylvie Brunet; Alain Moreau; Benoit Liandier; Damien Thierry; Françoise Cazein; Florence Lot; Caroline Semaille; Jean-Claude Desenclos is a Medicine article available to read on EtoBox.
What is Human immunodeficiency virus serotyping on dried serum spots as a screening tool for the surveillance of the AIDS epidemic about?
Many studies have demonstrated the utility of the dried blood spot (DBS) or dried plasma/serum spot (DSS) method for serological and molecular diagnosis of HIV infection. Here, we report on the description of a serotyping assay performed on DSS, and its application to a national surveillance program of HIV variants. We combined serotyping assays that we developed previously to discriminate between HIV-1 and HIV-2, between HIV-1 group O and HIV-1 group M, and between B and non-B subtypes of HIV-1 group M. The assays are based on antibody binding to either the immunodominant epitope of gp41 or the V3 domain of gp120 of these various types, groups and subtypes. Therefore, a unique enzyme-linked immunosorbent assay (ELISA) format applied to serum eluted from DSS allowed the simultaneous discrimination between infections caused by HIV-1 B, HIV-1 non-B, HIV-1 group O, and HIV-2. Together, this serotyping assay and an immunoassay for recent infection were used for a virological surveillance linked to the anonymous mandatory notification of HIV infection in France. The preliminary results of this virological surveillance allowed us to obtain estimates of the prevalence of the rare variants
Who reads Human immunodeficiency virus serotyping on dried serum spots as a screening tool for the surveillance of the AIDS epidemic?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Francis Barin; Jean-Christophe Plantier; Denys Brand; Sylvie Brunet; Alain Moreau; Benoit Liandier; Damien Thierry; Françoise Cazein; Florence Lot; Caroline Semaille; Jean-Claude Desenclos
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0146-6615)
- Published
- 2006
- Language
- EN
- Field
- Medicine (Health Sciences)