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This study investigates the role of the Clcn5 intronic regulatory element 3 (IRE3) using CRISPR/Cas9 and siRNA techniques in 3T3-L1 preadipocytes. The deletion of IRE3 resulted in significant upregulation of intronic miRNAs and altered expression of Clcn5 transcripts, indicating IRE3 functions as a regulatory switch for gene expression. These findings suggest that IRE3 plays a crucial role in modulating both miRNA transcription and alternative splicing of the Clcn5 gene.

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user-538188

Language

EN