Can I read Ginsenoside Rh1 protects human endothelial cells against lipopolysaccharide-induced inflammatory injury through inhibiting TLR2/4-mediated STAT3, NF-κB, and ER stress signaling pathways on EtoBox?

Ginsenoside Rh1 protects human endothelial cells against lipopolysaccharide-induced inflammatory injury through inhibiting TLR2/4-mediated STAT3, NF-κB, and ER stress signaling pathways by Yujin Jin; Thuy Le Lam Nguyen; Chang-Seon Myung; Kyung-Sun Heo is a Neuroscience article available to read on EtoBox.

What is Ginsenoside Rh1 protects human endothelial cells against lipopolysaccharide-induced inflammatory injury through inhibiting TLR2/4-mediated STAT3, NF-κB, and ER stress signaling pathways about?

## Aim: Endothelial cell (ec) dysfunction initiates atherosclerosis by inducing inflammatory cytokines and adhesion molecules. herein, we investigated the role of ginsenoside rh1 (rh1) in lipopolysaccharide (lps)-induced ec dysfunction. ## Main methods: The inhibitory effect of rh1 on lps binding to toll-like receptor 2 (tlr2) or tlr4 was evaluated using an immunofluorescence (if) assay. annexin v and cleaved caspase-3-positive ec apoptosis were evaluated by flow cytometry and if assay. western blotting and quantitative reverse transcription-pcr were performed to clarify underlying molecular mechanisms. in vivo model, effect of rh1 on ec dysfunction was evaluated by using en face if assay on aortas isolated c57bl/6 mice. ## Key finding: Lps (500 ng/ml) activated inflammatory signaling pathways, including erk1/2, stat3, and nf-κb. interestingly, rh1 significantly abolished the binding of lps to tlr2 and tlr4. consistently, rh1 inhibited lps-induced nf-κb activation and its downstream molecules, including inflammatory cytokines and adhesion molecules. furthermore, rh1 alleviated lps-induced downregulation of enos promoter activity. notably, inactivation of enos by 50 μm l-name signif

Who reads Ginsenoside Rh1 protects human endothelial cells against lipopolysaccharide-induced inflammatory injury through inhibiting TLR2/4-mediated STAT3, NF-κB, and ER stress signaling pathways?

Author

Yujin Jin; Thuy Le Lam Nguyen; Chang-Seon Myung; Kyung-Sun Heo

Publisher

Elsevier BV

Published

2022

Language

EN

Field

Neuroscience (Life Sciences)