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Can I read Biophysical and in-silico studies on the structure-function relationship of Brugia malayi protein disulfide isomerase on EtoBox?
Biophysical and in-silico studies on the structure-function relationship of Brugia malayi protein disulfide isomerase by Pawan Kumar Doharey; Pravesh Verma; Amit Dubey; Sudhir Kumar Singh; Manish Kumar; Timir Tripathi; Mona Alonazi; Nikhat Jamal Siddiqi; Bechan Sharma is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Biophysical and in-silico studies on the structure-function relationship of Brugia malayi protein disulfide isomerase about?
Human Lymphatic filariasis is caused by parasitic nematodes Wuchereria bancrofti, Brugia malayi, and Brugia timori. Protein disulfide isomerase (PDI), a redox-active enzyme, helps to form and isomerize the disulfide bonds, thereby acting as a chaperone. Such activity is essential for activating many essential enzymes and functional proteins. Brugia malayi protein disulfide isomerase (BmPDI) is crucial for parasite survival and an important drug target. Here, we used a combination of spectroscopic and computational analysis to study the structural and functional changes in the BmPDI during unfolding. Tryptophan fluorescence data revealed two well-separated transitions during the unfolding process, suggesting that the unfolding of the BmPDI is non-cooperative. The binding of the fluorescence probe 8-anilino-1-naphthalene sulfonic acid dye (ANS) validated the results obtained by the pH unfolding. The dynamics of molecular simulation performed at different pH conditions revealed the structural basis of BmPDI unfolding. Detailed analysis suggested that under different pH, both the global structure and the conformational dynamics of the active site residues were differentially altered. O
Who reads Biophysical and in-silico studies on the structure-function relationship of Brugia malayi protein disulfide isomerase?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Pawan Kumar Doharey; Pravesh Verma; Amit Dubey; Sudhir Kumar Singh; Manish Kumar; Timir Tripathi; Mona Alonazi; Nikhat Jamal Siddiqi; Bechan Sharma
- Publisher
- Informa UK Limited
- Published
- 2023
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)