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Characterization of mimotopes mimicking an immunodominant conformational epitope on the hepatitis C virus NS3 helicase by Colette Jolivet‐Reynaud; Anne Adida; Sandrine Michel; Gilbert Deléage; Glaucia Paranhos‐Baccala; Virginie Gonin; Nicole Battail‐Poirot; Xavier Lacoux; Dominique Rolland is a Medicine article available to read on EtoBox.
What is Characterization of mimotopes mimicking an immunodominant conformational epitope on the hepatitis C virus NS3 helicase about?
## Abstract The hepatitis C virus (HCV) nonstructural 3 (NS3) protein is composed of an amino terminal protease and a carboxyl terminal RNA helicase. NS3 contains major antigenic epitopes. The antibody response to NS3 appears early in the course of infection and is focused on the helicase region. However, this response cannot be defined by short synthetic peptides indicating the recognition of conformation‐dependent epitopes. In this study, we have screened a dodecapeptide library displayed on phage with anti‐NS3 mouse monoclonal antibodies (mAbs) that compete with each other and human anti‐HCV NS3 positive sera. Two peptides (mimotopes) were selected that appeared to mimic an immunodominant epitope since they were recognized specifically by the different anti‐NS3 mAbs of the study and by human sera from HCV infected patients. Homology search between the two mimotopes and the NS3 sequence showed that one of the two peptides shared amino acid similarities with NS3 at residues 1396–1398 on a very accessible loop as visualized on the three‐dimensional structure of the helicase domain whereas the other one had two amino acids similar to nearby residues 1376 and 1378. Reproduced as synt
Who reads Characterization of mimotopes mimicking an immunodominant conformational epitope on the hepatitis C virus NS3 helicase?
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- Author
- Colette Jolivet‐Reynaud; Anne Adida; Sandrine Michel; Gilbert Deléage; Glaucia Paranhos‐Baccala; Virginie Gonin; Nicole Battail‐Poirot; Xavier Lacoux; Dominique Rolland
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0146-6615)
- Published
- 2004
- Language
- EN
- Field
- Medicine (Health Sciences)