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The report investigates the enzyme kinetics of alkaline phosphatase, determining key parameters such as Vmax, Km, and Kcat using spectrophotometric techniques. The experiment utilized the Michaelis-Menten and Lineweaver-Burke models to analyze enzyme activity, yielding Vmax values of approximately 3.54 µM/min and Km values around 1.4585 mM. The findings confirm the reliability of the methods used and highlight the importance of enzyme kinetics in various scientific applications.

Author
sangal.kavya.01
Language
EN

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