Can I read On The Mechanism Of Drug Hydroxylation In Rat Liver Microsomes on EtoBox?

On The Mechanism Of Drug Hydroxylation In Rat Liver Microsomes by Sten Orrenius is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is On The Mechanism Of Drug Hydroxylation In Rat Liver Microsomes about?

The TPNH- and O~2~-dependent drug hydroxylation system of liver microsomes has been studied using normal rats and rats in which the drug-hydroxylating activity has been enhanced by repeated injections of phenobarbital. The oxidative demethylation of aminopyrine is employed as an assay. Optimal conditions for the assay with regard to the concentrations of TPNH and aminopyrine are established. TPN inhibits the reaction in a competitive manner, similarly to its effect on the microsomal TPNH-cytochrome __c__ reductase. Drug hydroxylation, but not the "TPNH oxidase," TPNH-cytochrome __c__, -2,6-dichlorophenolindophenol, or -neotetrazolium reductase reaction, or the TPNH-dependent lipid peroxidation, is blocked by carbon monoxide. Microsomes from phenobarbital-treated rats exhibit increased activities of the various TPNH-linked reductase reactions, parallel to the increased drug hydroxylation activity, whereas the "TPNH oxidase" activity does not change appreciably. Measurements with microsomes from drug-treated animals reveal a 1:1:1 stoichiometry of aminopyrine-dependent oxygen uptake, TPNH oxidation, and formaldehyde formation. Attempts to solubilize the drug-hydroxylating enzyme syst

Who reads On The Mechanism Of Drug Hydroxylation In Rat Liver Microsomes?

Author

Sten Orrenius

Publisher

Rockefeller University Press.; The Rockefeller University Press; Rockefeller University Press; ScienceOpen (ISSN 0021-9525)

Published

1965

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)