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Delineating Mechanisms of Dissociation for Isomeric Heparin Disaccharides Using Isotope Labeling and Ion Trap Tandem Mass Spectrometry by Ola M. Saad; Julie A. Leary is a Chemistry article available to read on EtoBox.
What is Delineating Mechanisms of Dissociation for Isomeric Heparin Disaccharides Using Isotope Labeling and Ion Trap Tandem Mass Spectrometry about?
Heparin and heparan sulfate (HS) glycosaminoglycans have been identified as important players in many physiological as well as pathophysiological settings. A better understanding of the biosynthesis and structure of these molecules is critical for further elucidation of their biological function. We have demonstrated the successful use of negative electrospray ionization tandem mass spectrometry in the differentiation of all twelve standard heparin-building blocks, including the potentially important N-unsubstituted disaccharides. Collision induced dissociation of each of the isomeric disaccharides provided unique product ion spectra, useful for identification and quantification of the relative amounts of each isomer present. In the research presented herein, isotopic labeling studies using (18)O and (2)H were used to determine the origins of each of the neutral losses observed in the product ion spectra, and mechanisms of dissociation consistent with the observed data were postulated. The general mechanisms postulated were for the generation of B, Y, and Z ions formed from glycosidic cleavages, as well as A and X ions formed from cross-ring cleavages. The eight isomeric heparin di
Who reads Delineating Mechanisms of Dissociation for Isomeric Heparin Disaccharides Using Isotope Labeling and Ion Trap Tandem Mass Spectrometry?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Ola M. Saad; Julie A. Leary
- Publisher
- Elsevier Science; Springer-Verlag; Springer New York LLC; American Chemical Society (ACS); Springer Science and Business Media LLC; Elsevier BV (ISSN 1044-0305)
- Published
- 2004
- Language
- EN
- Field
- Chemistry (Physical Sciences)