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A new approach to the study of Hodgkin lymphoma by flow cytometry by Maria Beatriz Álvarez Flores; María Sopeña Corvinos; María Medrano Élez; Beatriz Soto del Pecho; Luz Conejo Sánchez; Javier García de la Fe; Raquel Guillén Santos; Fernado Cava Valenciano is a Medicine article available to read on EtoBox.

What is A new approach to the study of Hodgkin lymphoma by flow cytometry about?

Hodgkin lymphoma (HL) appears to originate from germinal centre B cells but lacks expression of most B cell markers. In contrast to non-Hodgkin B lymphomas, HL is not routinely diagnosed using flow cytometry techniques, and diagnosis is mainly based on immunohistochemical and cytomorphological pathology studies. Hodgkin and Reed-Sternberg cells are large and fragile, making them difficult to study by flow cytometry. The aim of this study was to characterise the CD71 expression pattern on CD4+ T cells from HL patients and to design a simple flow cytometry algorithm to complement the histopathological diagnosis of HL. The present study suggests the utility of a conventional staining protocol with a simple panel of seven markers (CD15, CD30, CD4, CD8, CD71, CD3, and CD45) and a well-defined analysis strategy. The proposed algorithm uses the CD71 ratio (calculated as the percentage of CD71+ CD4+ T cells divided by the percentage of CD71+ CD45+ CD3- lymphocytes), with a cut-off of 0.5 to establish diagnosis groups as suggestive (≥0.5) or not suggestive (<0.5) of HL. In HL, CD71 expression is higher on CD4+ T lymphocytes than on non-T lymphocytes. In addition, the CD4+ T cell population

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Author

Maria Beatriz Álvarez Flores; María Sopeña Corvinos; María Medrano Élez; Beatriz Soto del Pecho; Luz Conejo Sánchez; Javier García de la Fe; Raquel Guillén Santos; Fernado Cava Valenciano

Publisher

Elsevier BV

Published

2023

Language

EN

Field

Medicine (Health Sciences)