Can I read Osteopontin Regulates Adhesion of Calcium Oxalate Crystals to Renal Epithelial Cells on EtoBox?

Osteopontin Regulates Adhesion of Calcium Oxalate Crystals to Renal Epithelial Cells by Takahiro Yasui; Keiji Fujita; Kiyofumi Asai; Kenjiro Kohri is a Medicine article available to read on EtoBox.

What is Osteopontin Regulates Adhesion of Calcium Oxalate Crystals to Renal Epithelial Cells about?

**Abstract** **__Background__**: The association of calcium crystals with renal tubular cells is an important factor during the formation of urinary stones. We previously reported the strong expression of osteopontin (OPN) on renal tubular cells in the stone‐forming kidney, suggesting that OPN plays a role in the crystal–cell interaction. In the present study, we examined the biological consequences of inhibiting OPN expression at the translational level on the formation and adhesion of crystals. **__Methods__**: We synthesized antisense OPN expression vector (pTet‐OPNas) using the tetracycline‐regulated expression system. The pTet‐OPNas was constructed using a mouse OPN cDNA sequence in an inverted (antisense) orientation. Two clones (NRK‐52E/ASs) were identified by transfection of pTet‐OPNas into NRK‐52E cells and they showed a marked reduction of OPN synthesis in the absence of tetracycline. Calcium oxalate (CaOx) crystal suspension was spread homogeneously on top of the NRK‐52E cells. After incubation, the association of CaOx crystals and cells was visualized by scanning electron microscopy. **__Results__**: Intact NRK‐52E cells, NRK‐52E cells transfected with empty vector and

Who reads Osteopontin Regulates Adhesion of Calcium Oxalate Crystals to Renal Epithelial Cells?

Author

Takahiro Yasui; Keiji Fujita; Kiyofumi Asai; Kenjiro Kohri

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0919-8172)

Published

2002

Language

EN

Field

Medicine (Health Sciences)