About this document
Comprehensive Guide to PCR Techniques by Hamza Khan is a document available to read on EtoBox.
PCR is a technique used to amplify a specific DNA sequence. It requires template DNA, reaction buffer, magnesium ions, dNTPs, primers, and DNA polymerase. Variations of PCR include colony PCR, nested PCR, and real-time PCR. Colony PCR is used to screen bacterial clones by picking a colony and using the boiled supernatant as a PCR template. Hot start PCR reduces non-specific amplification by inhibiting the polymerase until it is heated. Real-time PCR detects amplification as it occurs using fluorescent probe
- Author
- Hamza Khan
- Language
- EN