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Removal of nitrate and phosphate from simulated agricultural runoff water by Chlorella vulgaris 1 1. Introduction 2 2. Materials and methods 3 2.1. Microorganism and the culture method 3 2.2. Analytical methods 3 2.2.1. Measurement of algal biomass and cell concentration 3 2.2.2. Nitrate and phosphate concentrations 3 2.2.3. Protein content of cells 3 2.2.4. Total chlorophylls 4 2.2.5. DPPH radical scavenging assay 4 2.2.6. Determination of total phenolics 4 2.3. Statistical design of experiment and analysis of variance (ANOVA) 4 3. Results and discussion 5 3.1. Statistical analysis and ANOVAs of the models 5 3.2. Algal growth 5 3.3. Nitrate removal efficiency 9 3.4. Phosphate removal efficiency 9 3.5. Protein content of the microalga 13 3.6. Chlorophylls content of the microalga 15 3.7. Total phenolics in the microalga 17 3.8. Antioxidant levels in the microalga 18 4. Concluding remarks 18 CRediT authorship contribution statement 18 Declaration of competing interest 18 Acknowledgements 18 References 18