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Fetal Baboons Convert 18:3N-3 to 22:6N-3 in Vivo: a Stable Isotope Tracer Study by Hui-Min Su; Meng-Chuan Huang; Nabil M.R. Saad; Peter W. Nathanielsz; J. Thomas Brenna is a Medicine article available to read on EtoBox.

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Using [13C]-tracers and direct fetal doses, we show for the first time that the fetal primate converts alpha-linolenic acid (18:3) to docosahexaenoic acid (22:6) in vivo, and we estimate the relative bioefficacy of the two substrates for brain 22:6 accretion. Pregnant female baboons consumed a diet free of long chain polyunsaturates (LCP), with n-6/n-3 ratio of 10/1. In the third trimester of pregnancy (normal gestation = 182 days), they were instrumented with chronic indwelling catheters in the maternal femoral artery and the fetal jugular artery. Doses of either [U-13C]-18:3 (18:3\*, n = 3) or [U-13C]-22:6 (22:6\*, n = 2) were administered directly to the fetus. Blood was collected from fetus and mother, and the fetus was taken by cesarean section when electromyographic activity indicated that parturition was imminent. Fetal liver, brain, retina, and retinal pigment epithelium (RPE) were collected, and (13)C fatty acids determined. In 18:3\*- dosed animals, labeled n-3 LCP were detected in fetal plasma at 1 day post-dose and peaked at 2;-3 days; brain 22:6\* was constant at 3, 5, and 9 days post-dose, at 0.57 +/- 0.03 percent of dose (%Dose). In 22:6\*- dosed animals, brain 22:6\

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Author

Hui-Min Su; Meng-Chuan Huang; Nabil M.R. Saad; Peter W. Nathanielsz; J. Thomas Brenna

Publisher

Elsevier BV

Published

2001

Language

EN

Field

Medicine (Health Sciences)