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Determination of Phenolic Derivatives of Antipyrine in Plasma with Solid-phase Extraction and High-performance Liquid Chromatography–atmospheric-pressure Chemical Ionization Mass Spectrometry by Stefan A.J. Coolen; Thomas Ligor; Mark van Lieshout; Fred A. Huf is a Chemistry article available to read on EtoBox.

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This manuscript describes a method to determine antipyrine and its phenolic derivatives in plasma by reversed-phase high-performance liquid chromatography-mass spectrometry (RP-HPLC-MS). The sample pretreatment consisted of a C 18 solid-phase extraction (SPE), to remove the salts and proteins. The retention behavior of antipyrine and its phenolic derivatives in the SPE procedure was estimated by the k values determined on a C HPLC column at different pH values 18 and with different buffer compositions. Recoveries of antipyrine and its phenolic products were 90% in water and 100% in plasma. Atmospheric pressure chemical ionization (APCI) was used to introduce the components into the mass spectrometer. The mass spectrometer was operated in the single ion monitoring mode (SIM mode) as well as in the selective reaction (SR) mode. The SR mode or tandem MS resulted in the best signal-to-noise ratio, with a detection limit for antipyrine of 6 pg in 20 ml. For the different phenolic antipyrines, different target ions were used and conditions were optimized for each.

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Author

Stefan A.J. Coolen; Thomas Ligor; Mark van Lieshout; Fred A. Huf

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0378-4347)

Published

1999

Language

EN

Field

Chemistry (Physical Sciences)