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Label-free Multiplexed Virus Detection Using Spectral Reflectance Imaging by Carlos A. Lopez; George G. Daaboul; Rahul S. Vedula; Emre Özkumur; David A. Bergstein; Thomas W. Geisbert; Helen E. Fawcett; Bennett B. Goldberg; John H. Connor; M. Selim Ünlü is a Engineering article available to read on EtoBox.
What is Label-free Multiplexed Virus Detection Using Spectral Reflectance Imaging about?
We demonstrate detection of whole viruses and viral proteins with a new label-free platform based on spectral reflectance imaging. The Interferometric Reflectance Imaging Sensor (IRIS) has been shown to be capable of sensitive protein and DNA detection in a real time and high-throughput format. Vesicular stomatitis virus (VSV) was used as the target for detection as it is well-characterized for protein composition and can be modified to express viral coat proteins from other dangerous, highly pathogenic agents for surrogate detection while remaining a biosafety level 2 agent. We demonstrate specific detection of intact VSV virions achieved with surface-immobilized antibodies acting as capture probes which is confirmed using fluorescence imaging. The limit of detection is confirmed down to 3.5 × 10 5 plaqueforming units/mL (PFUs/mL). To increase specificity in a clinical scenario, both the external glycoprotein and internal viral proteins were simultaneously detected with the same antibody arrays with detergentdisrupted purified VSV and infected cell lysate solutions. Our results show sensitive and specific virus detection with a simple surface chemistry and minimal sample preparati
Who reads Label-free Multiplexed Virus Detection Using Spectral Reflectance Imaging?
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Carlos A. Lopez; George G. Daaboul; Rahul S. Vedula; Emre Özkumur; David A. Bergstein; Thomas W. Geisbert; Helen E. Fawcett; Bennett B. Goldberg; John H. Connor; M. Selim Ünlü
- Publisher
- Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0956-5663)
- Published
- 2011
- Language
- EN
- Field
- Engineering (Physical Sciences)