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Downregulation of P2X~7~ receptor expression in rat oligodendrocyte precursor cells after hypoxia ischemia by Li-Yan Wang; Wen-Qin Cai; Peng-Hui Chen; Qi-Yue Deng; Cong-Min Zhao is a Neuroscience article available to read on EtoBox.

What is Downregulation of P2X~7~ receptor expression in rat oligodendrocyte precursor cells after hypoxia ischemia about?

## Abstract Oligodendrocyte precursor cells (OPCs) are the predominant oligodendrocyte‐lineage stage in the cerebral hemispheres of neonatal rat. Prior studies have shown that OPCs are highly vulnerable to hypoxic‐ischemic injury, yet the mechanisms are not well understood. P2X~7~ receptor (P2X~7~R) is an ATP‐gated ion channel that has unusual properties and plays very complex roles in a variety of neuropathologic conditions. However, little is known about the involvement of P2X~7~R in OPCs development and injury. The present study was aimed at examining the presence of P2X~7~R in OPCs and evaluating the change of the receptor expression after hypoxia ischemia. Using Immunofluorescence, RT‐PCR, and western blot analysis, we demonstrated that OPCs expressed P2X~7~R __in vitro__ and __in vivo__. Activation of P2X~7~R in OPCs in response to 3′‐__O__‐(4‐benzoyl) benzoyl‐ATP (BzATP) led to an increased mobilization of intracellular calcium [Ca^2+^]i, formation of large pores and cell death. These functional responses were sensitive to pretreatment of cells with the P2X~7~R antagonist, Brilliant Blue G (BBG, 100 nM), which was a selective antagonist for P2X~7~R in nanomole range. A decre

Who reads Downregulation of P2X~7~ receptor expression in rat oligodendrocyte precursor cells after hypoxia ischemia?

Author

Li-Yan Wang; Wen-Qin Cai; Peng-Hui Chen; Qi-Yue Deng; Cong-Min Zhao

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0894-1491)

Published

2008

Language

EN

Field

Neuroscience (Life Sciences)