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Universal Sample Preparation Method for Proteome Analysis by Jacek R Wiśniewski; Alexandre Zougman; Nagarjuna Nagaraj; Matthias Mann is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Universal Sample Preparation Method for Proteome Analysis about?

We describe a method, filter-aided sample preparation (FASP), which combines the advantages of in-gel and in-solution digestion for mass spectrometry-based proteomics. We completely solubilized the proteome in sodium dodecyl sulfate, which we then exchanged by urea on a standard filtration device. Peptides eluted after digestion on the filter were pure, allowing single-run analyses of organelles and an unprecedented depth of proteome coverage.

Who reads Universal Sample Preparation Method for Proteome Analysis?

Author

Jacek R Wiśniewski; Alexandre Zougman; Nagarjuna Nagaraj; Matthias Mann

Publisher

Springer Science and Business Media LLC

Published

2009

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)