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Instant Determination of the Potential Biomarker Heparan Sulfate in Human Plasma by a Mix-and-read Fluorescence Assay by Warttinger, Ulrich; Krämer, Roland is a scholarly article available to read on EtoBox.

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Heparan sulfate (HS) is a linear, polydisperse sulfated polysaccharide belonging to the glycosaminoglycan family. HS proteoglycans are ubiquitously found at the cell surface and extracellular matrix in animal species. HS is involved in the interaction with a wide variety of proteins and the regulation of many biological activities. In certain pathologic conditions, expression and shedding of HS proteoglycans is overregulated, or enzymatic degradation of HS in lysosomes is deficient, both leading to excess circulating free HS chains in blood plasma. HS has therefore been suggested as a biomarker for various severe disease states. The structural heterogeneity makes the quantification of heparan sulfate in complex matrices such as human plasma challenging. HS plasma levels are usually quantified by either disaccharide analysis or enzyme linked immunosorbent assay(ELISA). Both methods require time-consuming multistep-protocols. We describe here the instant detection of heparan sulfate in spiked plasma samples by the Heparin Red Kit, a commercial mix-and-read fluorescence microplate assay. The method enables HS quantification in the low microgram per mL range without sample pretreatment

Author

Warttinger, Ulrich; Krämer, Roland

Published

2017

Language

EN