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Site-directed mutagenesis of the organ-specific element in the soybean leghemoglobin Ibc3 gene promoter by Karsten Bak Ramlov; Niels Bech Laursen; Jens Stougaard; Kjeld A. Marcker is a Agricultural and Biological Sciences article available to read on EtoBox.

What is Site-directed mutagenesis of the organ-specific element in the soybean leghemoglobin Ibc3 gene promoter about?

The expression of a soybean leghemoglobin 5′__Ibc__3–GUS‐3′__nos__ chimeric gene was analyzed in __Lotus corniculatus__ after site‐specific mutagenesis of the nodulin consensus sequences, AAAGAT and CTCTT, present in the organ‐specific element (OSE) (−139 to −102). Full‐length promoters (−1956,+46) carrying clustered point mutations in the CTCTT sequence or in both the AAAGAT and the CTCTT sequences were inactive. Point mutations in the AAAGAT sequence had only minor effects on the expression level. Substitution of the ATTG sequence between the AAAGAT and the CTCTT sequences in the OSE reduced the activity in nodules to 10%. This, together with the conservation of the ATTGT sequence in the same position of leg‐hemoglobin genes from other legumes, indicates that these sequences, in addition to the nodulin consensus sequences of the OSE, are important for high‐level nodule‐specific expression. Substitution of the CTCTT sequences outside the OSE (−44,−40 and −79,−75) results in promoter activities of approximately 50%.

Who reads Site-directed mutagenesis of the organ-specific element in the soybean leghemoglobin Ibc3 gene promoter?

Author

Karsten Bak Ramlov; Niels Bech Laursen; Jens Stougaard; Kjeld A. Marcker

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0960-7412)

Published

1993

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)