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Can I read A Disposable Biosensor for Urea Determination in Blood Based on an Ammonium-sensitive Transducer on EtoBox?
A Disposable Biosensor for Urea Determination in Blood Based on an Ammonium-sensitive Transducer by Claudia Eggenstein; Michael Borchardt; Christoph Diekmann; Bernd Gründig; Christa Dumschat; Karl Cammann; Meinhard Knoll; Friedrich Spener is a Engineering article available to read on EtoBox.
What is A Disposable Biosensor for Urea Determination in Blood Based on an Ammonium-sensitive Transducer about?
A potentiometric urea-sensitive biosensor using a NH4(+)-sensitive disposable electrode in double matrix membrane (DMM) technology as transducer is described. The ion-sensitive polymer matrix membrane was formed in the presence of an additional electrochemical inert filter paper matrix to improve the reproducibility in sensor production. The electrodes were prepared from one-side silver-coated filter paper, which is encapsulated for insulation by a heat-sealing film. A defined volume of the NH4(+)-sensitive polymer matrix membrane cocktail was deposited on this filter paper. To obtain the urea-biosensor a layer of urease was cast onto the ion-sensitive membrane. Poly (carbamoylsulfonate) hydrogel, produced from a hydrophilic polyurethane prepolymer blocked with bisulfite, served as immobilisation material. The disposable urea sensitive electrode was combined with a disposable Ag/AgCl reference electrode to obtain the disposable urea biosensor. The sensor responded rapidly and in a stable manner to changes in urea concentrations between 7.2 x 10(-5) and 2.1 x 10(-2)mol/l. The detection limit was 2 x 10(-5) mol/l urea and the slope in the linear range 52 mV/decade. By taking into con
Who reads A Disposable Biosensor for Urea Determination in Blood Based on an Ammonium-sensitive Transducer?
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Claudia Eggenstein; Michael Borchardt; Christoph Diekmann; Bernd Gründig; Christa Dumschat; Karl Cammann; Meinhard Knoll; Friedrich Spener
- Publisher
- Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0956-5663)
- Published
- 1999
- Language
- EN
- Field
- Engineering (Physical Sciences)