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Ligand-exchange radiochromoatographic resolution of [3H]dl-valine, [3H]dl-leucine and [3H]dl-methionine using a reverse-phase column in the presence of cupric acetate and GMP or cyanocobalamin by T. Fukuhara; M. Isoyama; M. Tanaka; S. Yuasa is a Physics and Astronomy article available to read on EtoBox.

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H]DL-Saline, ['H]DL-leucine and ['HIDI\_-methionine were resolved using ligand-exchange chromatography (reverse-phase) in the presence of cupric acetate [Cu(II)], and 5'-guanosine monophosphate or cyanocobalamin. The assignment of the resolved enantiomers was carried out by means of co-chromatography with non-labeled m-amino acids after modifying them with fluorodinitrobenzene. The optical purity of the enantiomers was estimated to be greater than 99%. The resolved enantiomers were subjected to bioassay, which showed that the enantiomers were biochemically active.

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Author

T. Fukuhara; M. Isoyama; M. Tanaka; S. Yuasa

Publisher

Elsevier Science; Elsevier ; Pergamon Press Ltd.; Elsevier BV (ISSN 0883-2889)

Published

1991

Language

EN

Field

Physics and Astronomy (Physical Sciences)