Opening book details…
Can I read Ozone Exposure Induces Metabolic Disorders and NAD+ Depletion Through PARP1 Activation in Spinal Cord Neurons on EtoBox?
Ozone Exposure Induces Metabolic Disorders and NAD+ Depletion Through PARP1 Activation in Spinal Cord Neurons by Ma, Shulin; Zhao, Xu; Zhang, Cong; Sun, Panpan; Li, Yun; Lin, Xiaowen; Sun, Tao; Fu, Zhijian is a Medicine article available to read on EtoBox.
What is Ozone Exposure Induces Metabolic Disorders and NAD+ Depletion Through PARP1 Activation in Spinal Cord Neurons about?
Background and Objective: Ozone therapy has shown therapeutic efficacy in different disorders particularly low back pain (LBP). However, ozone therapy has been associated with toxic effects on the respiratory, endocrine, cardiovascular systems as well as nervous system because of its strong oxidizing capacity. Recent studies have reported possible associations between ozone exposure and metabolic disorders, but the findings are controversial and little is known on the mechanisms of action. This study aims to investigate the cytotoxic effects of ozone exposure and possible mechanism of action in the animal model. Methods: Wistar neonate rats with the age of 24 h after birth were sacrificed by cervical dislocation under general anesthesia, then immersed in 75% alcohol and iodophor for 5 min, respectively. The spinal cord was isolated and cut to samples of ~1 mm^3^ and prepared for further experiments. The spinal cord neurons (SCNs) were exposed to ozone at different concentrations and then cultured in 96-well plates with glass bottom for 7 days. The cell viability, ATP levels and the NAD+ concentration were determined and compared between the different experimental groups and the con
Who reads Ozone Exposure Induces Metabolic Disorders and NAD+ Depletion Through PARP1 Activation in Spinal Cord Neurons?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Ma, Shulin; Zhao, Xu; Zhang, Cong; Sun, Panpan; Li, Yun; Lin, Xiaowen; Sun, Tao; Fu, Zhijian
- Publisher
- Frontiers Media SA; Frontiers Media S.A.; Lausanne: Frontiers Media S.A., 2014- (ISSN 2296-858X)
- Published
- 2020
- Language
- EN
- Field
- Medicine (Health Sciences)