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Enkephalins, dynorphins, and β‐endorphin in the rat dorsal horn: An immunofluorescence colocalization study by Juan Carlos G. Marvizón; Wenling Chen; Niall Murphy is a Neuroscience article available to read on EtoBox.

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## Abstract To characterize neuronal pathways that release opioid peptides in the rat dorsal horn, multiple‐label immunohistochemistry, confocal microscopy, and computerized co‐localization measures were used to characterize opioid‐containing terminals and cells. An antibody that selectively recognized β‐endorphin labeled fibers and neurons in the ventral horn as well as fibers in the lateral funiculus and lamina X, but practically no fibers in the dorsal horn. An anti‐enkephalin antibody, which recognized Leu‐, Met‐, and Phe‐Arg‐Met‐enkephalin, labeled the dorsolateral funiculus and numerous puncta in laminae I–III and V of the dorsal horn. An antibody against Phe‐Arg‐Met‐enkephalin, which did not recognize Leu‐ and Met‐enkephalin, labeled the same puncta. Antibodies against dynorphin and prodynorphin labeled puncta and fibers in laminae I, II, and V, as well as some fibers in the rest of the dorsal horn. Dynorphin and prodynorphin immunoreactivities colocalized in some puncta and fibers, but the prodynorphin antibody additionally labeled cell bodies. There was no co‐localization of dynorphin (or prodynorphin) with enkephalin (or Phe‐Arg‐Met‐enkephalin). Enkephalin immunoreactivit

Who reads Enkephalins, dynorphins, and β‐endorphin in the rat dorsal horn: An immunofluorescence colocalization study?

Author

Juan Carlos G. Marvizón; Wenling Chen; Niall Murphy

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0021-9967)

Published

2009

Language

EN

Field

Neuroscience (Life Sciences)