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Secreted phospholipase A~2~ potentiates glutamate‐induced calcium increase and cell death in primary neuronal cultures by Mark A. DeCoster; Gerard Lambeau; Michel Lazdunski; Nicolas G. Bazan is a Neuroscience article available to read on EtoBox.

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## Abstract Secreted phospholipases A~2~ (sPLA~2~s) modulate neuronal survival and neurotransmitter release. Here we show that sPLA~2~ (group III) synergistically increases glutamate‐induced cell death and intracellular calcium ([Ca^2+^]~i~) in cultured primary cortical and hippocampal neurons. Whereas 1 μM glutamate elicited transient [Ca^2+^]~i~ increases in all neurons that recovered 66% to baseline, 25 ng/ml sPLA~2~ pretreatment resulted in sustained [Ca^2+^]~i~ increases, with only 5% recovery. At 250 nM glutamate, 25% of neurons failed to respond, and the average recovery time was 101 ± 12 sec; sPLA~2~ increased recovery time to 158 ± 6 sec, and only 2% of cells failed to respond. Both the noncompetitive N‐methyl‐D‐aspartate (NMDA) receptor antagonist MK‐801 and the calcium‐channel blocker cobalt inhibited this effect. Experiments with the glutamate uptake inhibitor L‐__trans__‐pyrollidine‐2,4‐dicarboxylic acid (2.5 μM) indicated that glutamate uptake sites are not a likely modulation point by sPLA~2~, whereas arachidonic acid (AA) potentiated calcium responses to glutamate. Thus the enhancement of glutamate‐induced [Ca^2+^]~i~ increases by sPLA~2~ may be due to modulation at

Who reads Secreted phospholipase A~2~ potentiates glutamate‐induced calcium increase and cell death in primary neuronal cultures?

Author

Mark A. DeCoster; Gerard Lambeau; Michel Lazdunski; Nicolas G. Bazan

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0360-4012)

Published

2002

Language

EN

Field

Neuroscience (Life Sciences)