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α ‐L‐Rhamnosidase and β ‐D‐glucosidase activities in fungal strains isolated from alkaline soils and their potential in naringin hydrolysis by Dra. Lorena Alejandra Elíades; Natalia Lorena Rojas; Marta Noemí Cabello; Claudio Enrique Voget; Mario Carlos Nazareno Saparrat is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
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## Abstract __α__ ‐L‐Rhamnosidases (EC 3.2.1.40) and __β__ ‐D‐glucosidases (EC 3.2.1.21) obtained from several microbial sources are potential catalysts in food, beverage, and pharmaceutical industries. However, the enzyme preparations currently used have limitations related to the stability and activity of the enzyme as well to their reuse. A microtiter screening was carried out in 55 fungal strains isolated from alkaline soils, to obtain active __α__ ‐L‐rhamnosidases and __β__ ‐D‐glucosidases at pH 9.0. While __α__ ‐L‐rhamnosidase activity was detected in 45% of the strains tested, __β__ ‐D‐glucosidase activity was found only in 27%. Based on the fungal ability to produce __α__ ‐L‐rhamnosidase activity, cultures were supplemented with naringin to study the activities of the enzymes and the potential of the fungal strains on naringin hydrolysis. About 70% of the fungal strains tested increased the activities of both enzymes in the naringin‐supplemented cultures as compared to non‐supplemented ones. This effect was higher in __Acrostalagmus luteo‐albus__ LPSC 427 (15.3 fold) for __α__ ‐L‐rhamnosidase activity and __Metarrhizium anisopliae__ LPSC 996 (51.1 fold) for __β__ ‐D‐glucosi
Who reads α ‐L‐Rhamnosidase and β ‐D‐glucosidase activities in fungal strains isolated from alkaline soils and their potential in naringin hydrolysis?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Dra. Lorena Alejandra Elíades; Natalia Lorena Rojas; Marta Noemí Cabello; Claudio Enrique Voget; Mario Carlos Nazareno Saparrat
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Akademie Verlag; Wiley (ISSN 0233-111X)
- Published
- 2011
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)