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Genetic analysis of susceptibility to EAE in mice by O.A. Rossenwasser; J.K. O'Neill; E. Simpson; J.A. Todd; D. Baker is a Medicine article available to read on EtoBox.

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## Int~_etion -We ~_expre~_ 'gn of B7 Ag(S) ~ IL-10 by human adult microglia and ~ interaction with T cells. Materials and lVldlmds -BT/BB-1 Ag expression was a~\_tu~d in dissociated adult human gliM cell cultures using immunofluorescence and on froz¢~ sections from MS and control brains by immunocytochemistry. IL-10 production by microglia in vitro was assessed using RT-PCR and an IL-10 ELISA. An anti-BBl mAb, a CTLA-4 Ig fusion lm3te~, and rIL-10 and an anti-IL-10 Ab were used to assess B7 and IL-10 regulation ofT-cell responses. Results -Microgi/a but not astrocytes were B7/BB-l-positive in ~itro. Mioroggta in select MS brain lmions we~ also BT/BB-1positive. Microglia were positive for IL-10 mRNA and produced IL-10 following ~ ~mulation. Anti-B7 Abs ~d rIL-10 inhibited Ag presentation by microgLia in primary and secondary cell responses. Anti-IL-10 mAb augmented such responses. Conclusion -B7 Ags and IL-10 provide poteatial mechanisms for microglia to serve as immune regulatory cells within the CNS.

Who reads Genetic analysis of susceptibility to EAE in mice?

Author

O.A. Rossenwasser; J.K. O'Neill; E. Simpson; J.A. Todd; D. Baker

Publisher

Elsevier BV

Published

1994

Language

EN

Field

Medicine (Life Sciences)