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Clonality analysis of B-lymphoid proliferations using the polymerase chain reaction by Maria D. Lozano; Anne Tierens; Timothy C. Greiner; Robert S. Wickert; Dennis D. Weisenburger; Wing C. Chan is a Medicine article available to read on EtoBox.

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## BACKGROUND. Polymerase chain reaction (PCR) based assays are becoming more reliable, simpler, and faster alternatives to traditional Southern blot hybridization (SBH) analysis for the detection of clonal immunoglobulin heavy chain (IgH) gene rearrangements. However, a variety of technical approaches have been reported with markedly different results. ## METHODS. We analyzed the frozen tissue of 147 neoplastic and hyperplastic lesions on which SBH had previously been performed. Semi-nested and single-step PCR methods were compared. Consensus primers to the joining segments and the framework region (FR) 111 of the variable segments of the IgH gene were used. All PCR products were analyzed by polyacrylamide gel electrophoresis (PAGE). Thirteen samples were re-analyzed using a denaturing gradient gel electrophoresis (DGGE) system. ## RESULTS. The overall concordance between SBH and semi-nested PCR assays was 80.2%. In the non-Hodgkin's lymphoma (NHL) group, 75% of the cases with IgH rearrangements by SBH were found to be monoclonal by PCR. Regardless of type of lesion, 71.7% of the cases with IgH rearrangements by SBH were found to be clonal by PCR. The concordance between the semi-

Who reads Clonality analysis of B-lymphoid proliferations using the polymerase chain reaction?

Author

Maria D. Lozano; Anne Tierens; Timothy C. Greiner; Robert S. Wickert; Dennis D. Weisenburger; Wing C. Chan

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0008-543X)

Published

1996

Language

EN

Field

Medicine (Health Sciences)