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What is Erythrocyte Lipid Extraction Method about?
The document describes an improved procedure for extracting lipids from human red blood cells (erythrocytes) using chloroform-isopropanol in a 7:11 ratio. This procedure is simple, reproducible, extracts almost all of the cholesterol and phospholipids in a single extraction, uses one extraction tube, and yields an extract with only slight contamination from heme compared to other methods. It provides a more effective extraction of lipids from human erythrocytes than the commonly used Folch extraction method
- Author
- Sharif M Mizanur Rahman
- Language
- EN