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The document discusses the optimization and validation of a nicotinic acid analysis method using high performance liquid chromatography. The method was optimized by varying the mobile phase composition, flow rate, and extraction process. The optimum conditions included using a Kromasil column, mobile phase of 10 mM dipotassium hydrogen phosphate and potassium dihydrogen phosphate with 5 mM tetrabuthylammonium bromide at pH 7 and acetonitrile, flow rate of 0.8 ml/minute. The method was validated and showed a

Author

Naomita Joice

Language

EN