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In Vitro Synthesis of Oviductal Secretory Proteins by Estrogen‐treated Ovariectomized Gilts by W. C. Buhi; C. J. Ashworth; F. W. Bazer; I. M. Alvarez is a Environmental Science article available to read on EtoBox.

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## Abstract The objective of this study was to identify, characterize, and examine oviductal secretory proteins (OSP) synthesized de novo by whole oviduct (WO), ampulla (A), and isthmic (I) tissue from ovariectomized (OVX), corn oil (CO)‐, estrogen (E)‐, progesterone (P)‐, and E + P‐treated gilts. Oviducts were collected from OVX gilts after CO, E, P, or E + P treatment for 11 consecutive days and tissue was incubated with ^3^H‐leucine (^3^H‐leu). Rates of ^3^H‐leu incorporation into nondialyzable macromolecules by WO explants were greater (__P__ < 0.01) with E‐ compared to CO‐, P‐, or E + P‐treated gilts and greater (__P__ < 0.05) by A explants with E‐ compared to CO‐, P‐, or E + P‐treated gilts. An effect of location was noted, with A having a greater (__P__ < 0.01) rate of incorporation than WO or I. Conditioned culture medium was analyzed by one (1D)‐ and two‐dimensional (2D) sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) and fluorography. Analyses by 1D‐SDS‐PAGE revealed three major E‐dependent bands (335,000, 100,000, and 80,000 M~r~) in WO and A, and one (335,000 M~r~) in the I. A 20,000 M~r~ band found in A was inhibited by E, while a 60,000 M~r~ ban

Who reads In Vitro Synthesis of Oviductal Secretory Proteins by Estrogen‐treated Ovariectomized Gilts?

Author

W. C. Buhi; C. J. Ashworth; F. W. Bazer; I. M. Alvarez

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0022-104X)

Published

1992

Language

EN

Field

Environmental Science (Life Sciences)