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Enhancement in the perfection of orthorhombic lysozyme crystals grown in a high magnetic field (10 T) by Takao Sato; Yusuke Yamada; Shinya Saijo; Tetsuya Hori; Raita Hirose; Nobuo Tanaka; Gen Sazaki; Kazuo Nakajima; Noriyuki Igarashi; Masahiko Tanaka; Yoshiki Matsuura is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Enhancement in the perfection of orthorhombic lysozyme crystals grown in a high magnetic field (10 T) about?
Orthorhombic crystals of hen egg-white (HEW) lysozyme were grown in a homogeneous and static magnetic field of 10 T. All crystals grown at 10 T were oriented such that their crystallographic c axes were parallel to the magnetic field direction and showed a narrower average full-width at half-maximum (FWHM) of the rocking curve than those grown at 0 T. Rocking-width measurements were made at the BL-10A station at the Photon Factory, Tsukuba, Japan, using a high-resolution vertical-type four-circle diffractometer. Crystal perfection was evaluated using the FWHM of the rocking curve; the effects of the magnetic field on the quality of the crystals were examined by comparison of the FWHM of seven crystals grown at 10 and 0 T. The FWHMs of the reflections along the a, b and c axes decreased by 23.5, 35.3 and 27.8%, respectively, and those of other general reflections decreased by 17.4-42.2% in the crystals grown at high magnetic field. These results clearly showed that a magnetic field of 10 T improved the crystal perfection of the orthorhombic lysozyme crystals. As a result, the maximum resolution of X-ray diffraction increased from 1.3 A at 0 T to 1.13 A at 10 T. The magnetic field al
Who reads Enhancement in the perfection of orthorhombic lysozyme crystals grown in a high magnetic field (10 T)?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Takao Sato; Yusuke Yamada; Shinya Saijo; Tetsuya Hori; Raita Hirose; Nobuo Tanaka; Gen Sazaki; Kazuo Nakajima; Noriyuki Igarashi; Masahiko Tanaka; Yoshiki Matsuura
- Publisher
- International Union of Crystallography; Blackwell Publishing Inc.; International Union of Crystallography (IUCr) (ISSN 0907-4449)
- Published
- 2000
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)