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Modifying the helical structure of DNA by design: recruitment of an architecture-specific protein to an enforced DNA bend by Scot A. Wolfe; Ann E. Ferentz; Viara Grantcharova; Mair E.A. Churchill; Gregory L. Verdine is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Modifying the helical structure of DNA by design: recruitment of an architecture-specific protein to an enforced DNA bend about?

Proteins can force DNA to adapt distorted helical structures that are rarely if ever observed in naked DNA. The ability to synthesize DNA that cow tains defined helical aberrations would offer a new avenue for exploring the Ftructural and energetic plasticity of L1NA. Here WC report a strategy for the enforcement of non-canonical helical structures through diwltide cross-linking; this approach is exemplified by the design and s\-nthesi5 of an oligonuclcotide contmmig a pronounced bend. Results: A localized bend \vab cite-specifically introduced into I)NA by the formation of a disulfide crosslink bet\veen the 5' adenines of a 5'-AATT-3' region in con~plrmentary strands of L)NA. The DNA bend was characterized by high-resolution NMR structure determination of d croys-linked dodecamer and electrophoretic mobility assays on phased multirners, Lvhich together indicate that the cross-linked tetranucleotide induces a helical bend of -30 md a modest degree of unwinding. The enforced bend was found to stilnulate dramaticCdly the binding of an arc-hitecturespecific protein. HMG-I>. to the DNA. l>Nape I footprinting analysis revealed that the protein is recruited to the section of DNA that ig

Who reads Modifying the helical structure of DNA by design: recruitment of an architecture-specific protein to an enforced DNA bend?

Author

Scot A. Wolfe; Ann E. Ferentz; Viara Grantcharova; Mair E.A. Churchill; Gregory L. Verdine

Publisher

Elsevier Science; Elsevier ; Cell Press; Elsevier BV (ISSN 1074-5521)

Published

1995

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)