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Multi-oscillation Microrheology via Acoustic Force Spectroscopy Enables Frequency-dependent Measurements on Endothelial Cells at High-throughput† by Alfred Nguyen; Matthias Brandt; Till M. Muenker; Timo Betz is a Engineering article available to read on EtoBox.

What is Multi-oscillation Microrheology via Acoustic Force Spectroscopy Enables Frequency-dependent Measurements on Endothelial Cells at High-throughput† about?

Active microrheology is one of the main methods to determine the mechanical properties of cells and tissue, and the modelling of these viscoelastic properties is under heavy debate with many competing approaches. Most experimental methods of active microrheology such as optical tweezers or atomic force microscopy based approaches rely on single cell measurements, and thus suffer from a low throughput. Here, we present a novel method for frequency-dependent microrheology on cells using acoustic forces which allows multiplexed measurements of several cells in parallel. Acoustic force spectroscopy (AFS) is used to generate multi-oscillatory forces in the range of pN–nN on particles attached to primary human umbilical vein endothelial cells (HUVEC) cultivated inside a microfluidic chip. While the AFS was introduced as a single-molecule technique to measure mechanochemical properties of biomolecules, we exploit the AFS to measure the dynamic viscoelastic properties of cells exposed to different conditions, such as flow shear stresses or drug injections. By controlling the force and measuring the position of the particle, the complex shear modulus __G__\*(__ω__) can be measured continuou

Who reads Multi-oscillation Microrheology via Acoustic Force Spectroscopy Enables Frequency-dependent Measurements on Endothelial Cells at High-throughput†?

Author

Alfred Nguyen; Matthias Brandt; Till M. Muenker; Timo Betz

Publisher

Royal Society of Chemistry (RSC)

Published

2021

Language

EN

Field

Engineering (Physical Sciences)