Can I read A chemical method for introducing haptens onto DNA probes on EtoBox?
A chemical method for introducing haptens onto DNA probes by George H. Keller; Cecilia U. Cumming; Dao-Pei Huang; Mark M. Manak; Robert Ting is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is A chemical method for introducing haptens onto DNA probes about?
We have developed a versatile chemical method of attaching hapten moieties onto DNA, for the construction of nonisotopic DNA probes. The DNA is reacted with N-bromosuccinimide at alkaline pH, resulting in bromination of a fraction of the thymine, guanine, and cytosine residues, with adenine modified to a lesser extent. The bromine is subsequently displaced by a primary amino group, attached to a linker arm. The other end of the linker arm has a detectable group preattached to it. We have labeled cloned hepatitis B viral (HBV) DNA with the hapten 2,4-dinitrophenyl (DNP) and used it in combination with a high affinity rabbit anti-DNP antibody, for the detection of hepatitis B DNA by slot blotting. This probe was sensitive enough to specifically detect 1 X 10(-17) mol (1 X 10(6) copies) of HBV DNA in total DNA from human serum.
Who reads A chemical method for introducing haptens onto DNA probes?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- George H. Keller; Cecilia U. Cumming; Dao-Pei Huang; Mark M. Manak; Robert Ting
- Publisher
- Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)
- Published
- 1988
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)