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RNA with high template activity from leukaemic myeloblasts (Bai strain a virus-induced avian myeloblastosis) I. Optimal conditions for its isolation and activity in the subcellular protein-synthesizing system by M. Trávníč; L. Veprek; J. Ríman is a Medicine article available to read on EtoBox.

What is RNA with high template activity from leukaemic myeloblasts (Bai strain a virus-induced avian myeloblastosis) I. Optimal conditions for its isolation and activity in the subcellular protein-synthesizing system about?

Three methods of phenol fractionation of metabolically active R NA of leukaemic myeloblasts were compared on the basis of the template activity of the isolated jractions. It was shown that the method of phenol fractionation based on increasing the extraction temperature is most suitable for obtaining a fraction substantially enriched in template active RNA. Standardization of the extraction conditions employed (the use of acetate bufler pH 6 ) led to the separation of RNA of high template activity (6S0-RNA) not contaminated with D N A arid highly purified with respect to the presence of other cellular R NAs. The template activity of isolated R N A fractions was tested in the subcellular proteinsynthesizing system from E. coli under the optimal conditions described here with respect to the function of the R N A fraction with the highest template activity It was found that the 6.50-R NA fraction has characteristic sedimentation properties, a nucleotide composition resembling the D N A of Ieukaemic myeloblctsts and, by its high template activity, meets the requirements of a highly purijiedpreparation of m R N A . Its template activity, tested by incorporation of two amino acids (2160

Who reads RNA with high template activity from leukaemic myeloblasts (Bai strain a virus-induced avian myeloblastosis) I. Optimal conditions for its isolation and activity in the subcellular protein-synthesizing system?

Author

M. Trávníč; L. Veprek; J. Ríman

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)

Published

1967

Language

EN

Field

Medicine (Health Sciences)