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Liposomes modified with polycation used for gene delivery: Preparation, characterization and transfection in vitro by Jin-Liang Chen; Hua Wang; Jian-Qing Gao; Hai-Liang Chen; Wen-Quan Liang is a Pharmacology, Toxicology and Pharmaceutics article available to read on EtoBox.
What is Liposomes modified with polycation used for gene delivery: Preparation, characterization and transfection in vitro about?
Gene therapy provides great opportunities for treating diseases from genetic disorders, infections and cancer. The development of efficient and safe gene transfer systems could be one of the most important factors for successful gene therapy. In the present study, an amphiphilic compound, polyethylenimine (PEI, MW 800)-cholesterol (PEI 800-Chol), firstly designed to modify the surface of liposomes, was synthesized. Polycation liposomes (PCLs) composed of soybean phospholipids (SPL), cholesterol (Chol) and PEI 800-Chol were prepared using film hydration method. The mean particle size of the PCLs was 133.0 nm and the zeta potential was 50.1 ± 2.6 mV. Due to the PEI anchored onto the surface of liposomes, higher buffering capacity of PCLs was observed, indicating the potential for buffering in the acidic pH environment of the endosomes. Compared to Lipofectamine TM 2000, PCLs have equivalent transfection efficiency with significantly low cytotoxicity. Interestingly, the transfection activity of PCLs was not influenced in the presence of serum. Furthermore, we constructed another PCL composed of PEI 800-Chol and DOPE, and transfection efficiency increased notably. In conclusion, the PC
Who reads Liposomes modified with polycation used for gene delivery: Preparation, characterization and transfection in vitro?
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- Author
- Jin-Liang Chen; Hua Wang; Jian-Qing Gao; Hai-Liang Chen; Wen-Quan Liang
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0378-5173)
- Published
- 2007
- Language
- EN
- Field
- Pharmacology, Toxicology and Pharmaceutics (Life Sciences)