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Can I read Relationship Between Body Conditions and Environmental Contaminants in Bovine Ovarian Cells on EtoBox?
Relationship Between Body Conditions and Environmental Contaminants in Bovine Ovarian Cells by Sirotkin, Alexander V.; Makarevich, Alexander V.; Kubovicova, Elena; Medvedova, Marina; Kolesarova, Adriana; Harrath, Abdel Halim is a Medicine article available to read on EtoBox.
What is Relationship Between Body Conditions and Environmental Contaminants in Bovine Ovarian Cells about?
The effect of body condition and environmental contaminants on reproductive processes is known; however, it is not known whether basic ovarian cell functions and their response to these contaminants depend on body condition. This study aimed to understand the interrelationships between body conditions and environmental contaminants on ovarian cells. For this purpose, we compared ovarian granulosa cells isolated from cows with an emaciation tendency (body condition score, BCS2 on a scale from 1 to 5) and cows with average body condition (BCS3); proliferation, apoptosis, secretory activity and the response to environmental contaminants were all assessed in the cells. In the 1st series of experiments, ovarian granulosa cells isolated from BCS2 and BCS3 cows were cultured with and without benzene, xylene, and toluene (0.1%). The accumulation of nuclear and cytoplasmic markers of apoptosis (p53 and bax, respectively), a proliferation marker (PCNA), progesterone (P4), and insulin-like growth factor I (IGF-I) was evaluated by Western blot and radioimmunoassay (RIA) experiments. In the 2nd series of experiments, the groups of granulosa cells were cultured with and without mycotoxine deoxyn
Who reads Relationship Between Body Conditions and Environmental Contaminants in Bovine Ovarian Cells?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Sirotkin, Alexander V.; Makarevich, Alexander V.; Kubovicova, Elena; Medvedova, Marina; Kolesarova, Adriana; Harrath, Abdel Halim
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0093-691X)
- Published
- 2020
- Language
- EN
- Field
- Medicine (Health Sciences)