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Partial characterization of bacteriocins produced by environmental strain Enterococcus faecium EK13 by M. Mareková; A. Lauková; L. DeVuyst; M. Skaugen; I.F. Nes is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Aims: The partial characterization of bacteriocins produced by an environmental strain enterococcus faecium ek13, isolated from cattle dung water. ## Methods and results: A bacteriocin was partially purified by ammonium sulphate precipitation, followed by a sp-sepharose column, reverse-phase chromatography and n-terminal region sequenced. the anti-microbial substance produced was found to be a heat-stable polypeptide with molecular mass 4.83 kda, which was determined by n-terminal amino acid sequencing to be enterocin a. a second substance was specified by pcr as enterocin p. bacteriocins were stable at 4 and -20 degrees c for long storage periods. the optimum of bacteriocin production was observed in the range of ph 5.0-6.5 at 30 and 37 degrees c. the most active substances are produced by strain ek13 in logarithmic growth phase and bacteriocins are produced after 1 h of fermentation. the highest activity detected in fermentation experiments was 51 200 au ml(-1) and the most sensitive indicator strain was found to be listeria innocua lmg 13568. differences in bacteriocin activity against two indicators could be explained by more than one type of enterocin production by strain e

Who reads Partial characterization of bacteriocins produced by environmental strain Enterococcus faecium EK13?

Author

M. Mareková; A. Lauková; L. DeVuyst; M. Skaugen; I.F. Nes

Publisher

Oxford University Press (OUP)

Published

2003

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)