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Can I read Small circular DNA in Drosophila melanogaster on EtoBox?
Small circular DNA in Drosophila melanogaster by Sharon Stanfield; Donald R. Helinski is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Small circular DNA in Drosophila melanogaster about?
Covalently closed small circular DNA isolated from Drosophila melanogaster is described . The small circular DNA is found in blastema stage eggs and in Schneider's cell culture line 2 and a cloned subline of line 2 . It is heterogeneous in size, although the size distributions and mean sizes differ for each source. The small circular DNA from Schneider's line 2cells ranges from 0 .09-7 .3μm, with a mean contour length of 1 .1 μm . This DNA has a buoyant density of 1 .703 g/cc and appears to be present predominantly in the nuclear fraction of detergent-disrupted cells . The restriction enzyme EcoRl cleaves approximately 40% of the small circular DNA with a bias toward the larger size classes . Both logarithmic and stationary phase cells contain approximately 3-40 average sized small circular DNA molecules per cell, representing a maximum of 0 .03% of the total cellular DNA . Exposure to cycloheximide or puromycin for 14 hr results in a 30 fold increase in the number of small circles per cell, but reduces the mean length of the circular DNA to 0 .3 μm . The drug-amplified DNA has a buoyant density in the range of 1 .698-1 .703 g/cc. No amplification was seen in cells treated with eit
Who reads Small circular DNA in Drosophila melanogaster?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Sharon Stanfield; Donald R. Helinski
- Publisher
- Elsevier BV
- Published
- 1976
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)