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DNA replication occurs through a semiconservative mechanism where the two parental DNA strands separate and each serves as a template for a new progeny strand. Experiments using labeled E. coli DNA provided evidence for this semiconservative model. DNA replication can also occur through a semidiscontinuous mechanism, as shown by the discovery of Okazaki fragments in T4 DNA replication. DNA replication requires several enzymes including DNA polymerases, helicases, single-stranded DNA binding proteins, topois

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