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Carbon Dioxide Improves the Growth of Hairy Roots Cultured on Solid Medium and in Nutrient Mists by Alexander A. DiIorio; Ronald D. Cheetham; Pamela J. Weathers is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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The effect of varying C O 2 concentrations on the growth of beet and safflower hairy roots was measured for tissues cultured in nutrient mists and on solid media in chambers fed mixtures of humidified air supplemented with different CO2 concentrations. Hairy root tissue grown on solid media in air enriched with CO2 showed increased growth, as measured by dry weight increases vs air-fed controls. Growth increased with COz enrichment as much as 2.5 times more than the air-fed control for safflower at 1.0070 CO2 and 1.4 times more than the air-fed control for beets at 1.5070 CO2 over a 12-day period. Beet hairy root tissue was also cultured aeroponically in nutrient mists. Beet hairy root cultured in nutrients mists enriched with 1.0% CO2 showed a 1507o increase in biomass over a 7-day period vs tissue cultured in nutrient mists (with ambient air) or in shake flasks. The stimulation of root growth via CO2 enrichment reduced the time required for biomass accumulation.

Who reads Carbon Dioxide Improves the Growth of Hairy Roots Cultured on Solid Medium and in Nutrient Mists?

Author

Alexander A. DiIorio; Ronald D. Cheetham; Pamela J. Weathers

Publisher

Springer; Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1432-0614)

Published

1992

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)